Journal articles
Analysis of high-molecular-weight proteins using MALDI-TOF MS and machine learning for the differentiation of clinically relevant Clostridioides difficile ribotypes. Ana Candela, David Rodriguez-Temporal, Mario Blázquez-Sánchez, Manuel J. Arroyo, Mercedes Marín, Luis Alcalá, Germán Bou, Belén Rodríguez-Sánchez & Marina Oviaño. European Journal of Clinical Microbiology & Infectious Diseases, Dec 2024. 
Multicenter evaluation of Fourier transform infrared (FTIR) spectroscopy as a first-line typing tool for carbapenemase-producing Klebsiella pneumoniae in clinical settings. Ana Candela, David Rodríguez-Temporal, Pilar Lumbreras, Paula Guijarro-Sánchez, Manuel J. Arroyo, Fernando Vázquez, Alejandro Beceiro, Germán Bou, Patricia Muñoz, Marina Oviaño, Javier Fernández, Belen Rodriguez-Sánchez. Journal of Clinical Microbiology, November 2024.
Neisseria leonii sp. nov., isolated from the nose, lung, and liver of rabbits. Martin Boutroux, Sandrine Favre-Rochex, Olivier Gorgette, Gérald Touak, Estelle Mühle, Christiane Bouchier, Olivier Chesneau, Frédéric J. Veyrier, Dominique Clermont and Praveen Rahi. International Journal of systematic and evolutionary microbiology, Accepted. July 2024.
Development and validation of a quick, automated and reproducible ATR-FTIR spectroscopy machine learning model for Klebsiella pneumoniae typing. Ângela Novais, Ana Beatriz Gonçalves, Teresa Ribeiro, Ana Freitas, Gema Méndez, Luis Mancera, Antonia Read, Valquiria Alves, Lorena López-Cerero, Jesús Rodríguez-Baño, Alvaro Pascual, and Luísa Peixe. Journal of Clinical Microbiology, Accepted. Dec 2023.
Discrimination between hypervirulent and non-hypervirulent ribotypes of Clostridioides difficile by MALDI-TOF mass spectrometry and machine learning. Ahmed Mohamed Mostafa Abdrabou, Issa Sy, Markus Bischoff, Manuel J. Arroyo, Sören L. Becker, Alexander Mellmann, Lutz von Müller, Barbara Gärtner, Fabian K. Berger. European Journal of Clinical, Sep 2023.
Rapid Discrimination of Pseudomonas aeruginosa ST175 Isolates Involved in a Nosocomial Outbreak Using MALDI-TOF Mass Spectrometry and FTIR Spectroscopy Coupled with Machine Learning. Ana Candela, Manuel Arroyo, María Sánchez-Cueto, Mercedes Marín, Emilia Cercenado, Gema Méndez, Patricia Muñoz, Luis Mancera, David Rodriguez-Temporal and Belén Rodríguez-Sánchez. Transboundary and Emerging Diseases, Sep. 2023.
Direct detection of carbapenemase-producing Klebsiella pneumoniae by MALDI-TOF analysis of full spectra applying machine learning. Eva Gato, Manuel J. Arroyo, Gema Méndez, Ana Candela, Bruno K Rodiño-Janeiro, Javier Fernández, Belen Rodriguez, Luis Mancera, Jorge Arca-Suárez, Alejandro Beceiro, Germán Bou, and Marina Oviaño. Journal of Clinical Microbiology, May 2023.
Evaluation of Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry for Molecular Typing of Acinetobacter baumannii in Comparison with Orthogonal Methods. Eloise J. Busby, Ronan M. Doyle, Clara Leboreiro Babe, Kathryn A. Harris, Damien Mack, Gema Méndez-Cervantes, Denise M. O’Sullivan, Vicky Pang, Zahra Sadouki, Priya Solanki, Jim F. Huggett, Timothy D. McHugh, Emmanuel Q. Wey. Journal of Clinical Microbiology, May 2023.
Automatic discrimination of species within the Enterobacter cloacae complex using MALDI-TOF Mass Spectrometry and supervised algorithms. A. Candela, A. Guerrero-Lopez, M. Mateos, A. Gomez Asenjo, M.J. Arroyo, M. Hernández-García, R. del Campo, E. Cercenado, A. Cuenod, G. Méndez, L. Mancera, J. de Dios Caballero, L. Martínez-García, D. Gijón, M.I. Morosini, P. Ruiz-Garbajosa, A. Egli, R. Cantón, P. Muñoz, D. Rodriguez-Temporal, B. Rodriguez-Sanchez. Journal of Clinical Microbiolog, Feb. 2023.
Identification of Mycobacterium abscessus subspecies by MALDI-TOF Mass Spectrometry and Machine Learning. D. Rodríguez-Temporal, L. Herrera, F. Alcaide, D. Domingo, G. Héry-Arnaud, J. van Ingen, A. Van den Bossche, A. Ingebretsen, C. Beauruelle, E. Terschlüsen, S. Boarbi, N. Vila-Olmo, M.J. Arroyo, G. Méndez, P. Muñoz, L. Mancera, M.J. Ruiz-Serrano, B. Rodríguez-Sánchez. Journal of Clinical Microbiology, Jan. 2023.
Ultrasensitive and rapid identification of ESRI developer- and piperacillin/tazobactam-resistant Escherichia coli by the MALDIpiptaz tests. A. Rodríguez-Villodres, L. Gálvez-Benítez, M.J. Arroyo, G. Méndez, L. Mancera, A. Vila-Domínguez, J.A. Lepe-Jiménez, Y. Smani. Emerging Microbes & Infections, Aug. 2022.
Rapid and reproducible MALDI-TOF-based method for the detection of Vancomycin-resistant Enterococcus faecium using classifying algorithms. A. Candela, M.J. Arroyo, A. Sánchez-Molleda, G. Méndez, L. Quiroga, A. Ruiz, E. Cercenado, M. Marín, P. Muñoz, L. Mancera, D. Rodríguez-Temporal, B. Rodríguez-Sánchez. Diagnostic Microbiology and Infectious Disease, Jan. 2022.
Detection of azole resistance in Aspergillus fumigatus complex isolates using MALDI-TOF mass spectrometry. M.E. Zvezdanova, M.J. Arroyo, G. Méndez, A. Candela, L. Mancera, J. García, J. Lozano, R. Jiménez, I. Lozano, C. Castro, C. López, P. Muñoz, J. Guinea, P. Escribano. Clinical Microbiology and Infection, Feb. 2022.
Multicenter Performance Evaluation of MALDI-TOF MS for Rapid Detection of Carbapenemase Activity in Enterobacterales: The future of Networking Data Analyisis With Online Software. Gato E, Anantharajah A, Arroyo MJ, Artacho MJ, Caballero JdD, Candela A, Chudějová K, Constanso IP, Elías C, Fernández J, Jiménez J, Lumbreras P, Méndez G, Mulet X, Pérez-Palacios P, Rodríguez-Sánchez B, Cantón R, Hrabák J, Mancera L, Martínez-Martínez L, Oliver A, Pascual Á, Verroken A, Bou G and Oviaño M. Frontiers in Microbiology, Jan. 2022.
Occurrence of the p019 Gene in the blaKPC-Harboring Plasmids: Adverse Clinical Impact for Direct Tracking of KPC-Producing Klebsiella pneumoniae by Matrix-Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry. E. Gato, I. P. Constanso, B. Kotska Rodiño-Janeiro, P. Guijarro-Sánchez, T. Alioto, M.J. Arroyo, G. Méndez, L. Mancera, M. Gut, I. Gut, M. Álvarez.-Tejado, G. Bou, M. Oviaño. Journal of Clinical Microbiology, Jul. 2021.
An Improved Matrix-Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry Data Analysis Pipeline for the Identification of Carbapenemase-Producing Klebsiella pneumoniae. E. Gato, I. P. Constanso, A. Candela, F. Galán, B. K. Rodiño-Janeiro, M. J. Arroyo, G. Méndez, L. Mancera, T. Alioto, M. Gut, I. Gut, M. Álvarez-Tejado, B. Rodríguez-Sánchez, G. Bou, M. Oviaño. Journal of Clinical Microbiology, Jun. 2021.
Implementation of MALDI-TOF Mass Spectrometry and Peak Analysis: Application to the Discrimination of Cryptococcus neoformans Species Complex and Their Interspecies Hybrids. M.E. Zvezdanova, M.J. Arroyo, G. Méndez, J.V. Guinea Ortega, L. Mancera, P. Muñoz, B. Rodríguez-Sánchez, P. Escribano. Journal of Fungi, Dec. 2020.
MALDI-MS Protein Profiling of Chemoresistance in Extracellular Vesicles of Cancer Cells. G. Stübiger, M.D. Nairn, T.K. Abban, M.E. Openshaw, L. Mancera, B. Herzig, M. Wuczkowski, D. Senfter, R.M. Mader. Analytical Chemistry, Nov. 2018.
Characterization of Yeasts and Filamentous Fungi using MALDI Lipid Phenotyping. G. Stübiger, M.D. Nairn, T.K. Abban, M.E. Openshaw, L. Mancera, B. Herzig, M. Wuczkowski, D. Senfter, R.M. Mader. Journal of Microbiological Methods, Nov. 2016.
Book chapters
Machine Learning in Analysis of Complex Flora Using Mass Spectrometry. L. Mancera, Manuel J. Arroyo, G. Méndez, O. Belgacem, B. Rodriguez-Sanchez, M. Oviaño. Microbiological Identification using MALDI-TOF and Tandem Mass Spectrometry: Industrial and Environmental Applications. Eds. Haroun N. Shah, Saheer E. Gharbia, Ajit J. Shah, Erika Y. Tranfield, K. Clive Thomson. Wiley, April 2023.
International conferences
Direct Saliva Analysis by MALDI Mass Spectrometry: A Workflow Suitable for Future Clinical Applications | Poster Communication | 5th Annual Congress Liquid Biopsy | L.Sellami1 , J. Jiménez2, O. Belgacem1 | 1Ascend Diagnostics LTD, 2Clover Biosoanalytical Software.
Detección rápida de la sensibilidad a imipenem/relebactam y predicción de mecanismos de resistencia en Pseudomonas aeruginosa mediante espectrometría de masas MALDI-TOF | Oral communication | XXVI Congreso de la Sociedad Española de Enfermedades Infecciosas y Microbiología Clínica (SEIMC 2023) | A. Candela1 , M. Fernández-Billón2, I. Alonso1, P. Fraile Ribot2, C. López Causapé2, M.J. Arroyo3, L. Mancera3, A. Beceiro1, G. Bou1, A. Oliver2, J. Arca Suárez1, M. Oviaño1 | 1Complexo Hospitalario Universitario de A Coruña, 2Hospital Universitario Son Espases, 3Clover Biosoanalytical Software.
Aplicación clínica de la espectrometría de masas MALDI-TOF para la diferenciación de Mycobacterium chimaera y Mycobacterium intracellulare | Oral communication | XXVI Congreso de la Sociedad Española de Enfermedades Infecciosas y Microbiología Clínica (SEIMC 2023) | A. Candela1 , D. Rodríguez Temporal2 , M. Fernández-Billón3, M.J. Arroyo4, M. Marín2, L. Mancera4, G. Bou1 , B. Fernández Pérez1, B. Rodríguez Sánchez2, M. Oviaño1 | 1Complexo Hospitalario Universitario de A Coruña, 2Hospital General Universitario Gregorio Marañón, 3Hospital Universitario Son Espases, 4Clover Biosoanalytical Software.
Desarrollo de modelos de clasificación automática basados en espectrometría de masas MALDI-TOF y Machine Learning para el tipado rápido de Clostridioides difficile | Oral communication | XXVI Congreso de la Sociedad Española de Enfermedades Infecciosas y Microbiología Clínica (SEIMC 2023) | D. Rodriguez-Temporal1, M. Blázquez-Sánchez1, A. Guerrero-López2, A. Candela3, L. Jiménez-Rosillo1, M. Sánchez-Cueto1, M. J. Arroyo4, A. Martín1, L. Jiménez-Navarro1, V. Gómez-Verdejo2, P. M. Olmos2, G. Méndez4, L. Mancera4, P. Muñoz1, L. Alcalá1, M. Marín1, B. Rodríguez-Sánchez1 | 1Servicio de Microbiología y Enfermedades Infecciosas, Hospital General Universitario Gregorio Marañón, 2Departamento de Teoría de la Señal y Comunicaciones, Universidad Carlos III, 3Servicio de Microbiología, Complejo Hospital Universitario A Coruña, 4Clover Biosoanalytical Software.
High Molecular Weight Differentiation of Carbapenemase-Producing Klebsiella Pneumoniae isolates using MALDI-TOF MS | Oral communication | Asturias International Meeting on Clinical Microbiology and Infectious Diseases (AIMID 2022) | Ana Candela1, Manuel J. Arroyo2, Gema Méndez2, Luis Mancera2, German Bou1, Marina Oviaño1 | 1Complexo Hospitalario Universitario A Coruña (CHUAC), 2Clover Bioanalytical Software, Granada, Spain.
Turning ATR FT-IR spectroscopy into an easy, quick and reproducible Klebsiella pneumoniae | Oral communication | 32nd European Congress of Clinical Microbiology & Infectious Diseases (ECCMID 2022) | Â. Novais1* (Porto, Portugal), A.B. Gonçalves1, T.G. Ribeiro1, A.R. Freitas1,2, G. Mendéz3, L. Mancera3, A. Read4, V. Alves4, L. López-Cerero5, J. Rodríguez- Baño5, Á. Pascual5, L. Peixe1 | 1UCIBIO/REQUIMTE and Associate Laboratory i4HB – Institute for Health and Bioeconomy. Laboratory of Microbiology Faculty of Pharmacy University of Porto; 2TOXRUN, Toxicology Research Unit, University Institute of Health Sciences, CESPU, CRL, Gandra, Portugal; 3Clover Bioanalytical Software, Granada, Spain; 4Hospital Pedro Hispano, Porto, Portugal, 5Hospital Vírgen Macarena, Seville, Spain.
Rapid Clostridiodes difficile ribotyping based on MALDI-TOF MS and machine learning | Oral communication | 32nd European Congress of Clinical Microbiology & Infectious Diseases (ECCMID 2022) | A. Candela1,2* (A Coruña, Spain), D. Rodriguez-Temporal1,2, M.J. Arroyo3, M. Marin1,2,4,5 A. Martin1, L. Jimenez-Navarro1, L. Alcalá1,2 G. Mendez3, P. Muñoz1,2,4,5 L. Mancera3, B. Rodriguez-Sanchez1,2 | 1Clinical Microbiology and Infectious Diseases Department, Hospital General Universitario Gregorio Marañón, Madrid, Spain; 2Instituto de Investigación Sanitaria, Hospital Gregorio Marañón, Madrid, Spain; 3Clover Bioanalytical Software, Granada, Spain, 4Department of Medicine, School of Medicine, Universidad Complutense de Madrid, Madrid, Spain; 5CIBER Enfermedades Infecciosas CIBERES (CB06/06/0058), Spain.
MALDI-nanochip based Screening of Exosomal Biomarkers: Application to Cancer Diagnostics | American Society for Mass Spectrometry (ASMS 2020) | Michael D. Nairn1, Michael Wuczkowsky2, Jesús Jiménez3, Iris Prinz4, Marco Rissoglio5, Gerald Stubiger6 | 1Shimadzu, Manchester, UK. 2Medical University of Vienna, Vienna, Austria. 3Clover Bioanalytical Software, Granada, Spain. 4Stratec Consumables, Salzburg, Austria.
Rapid MALDI-TOF MS-based method for vancomycin-resistant Enterococcus faecium detection | 30th European Congress of Clinical Microbiology & Infectious Diseases (ECCMID 2020) | Ana Candela1, Lidia Quiroga1, Manuel J. Arroyo2, Adrián Ruiz1, Emilia Cercenado1, Gema Méndez2, Mercedes Marín1, Patricia Muñoz1, Luis Mancera2, Belén Rodríguez-Sánchez1 | 1Hospital General Universitario Gregorio Marañón, Madrid, Spain. 2Clover Bioanalytical Software, Granada, Spain.
Accurate differentiation of carbapenemases by MALDI-TOF MS-typing: employment of bioinformatics | 30th European Congress of Clinical Microbiology & Infectious Diseases (ECCMID 2020) | Eva Gato1, Bruno Kotska Rodiño1, Gema Méndez2, Manuel J. Arroyo2, Luis Mancera2, Germán Bou1, Marina Oviaño1 | 1Clinical Microbiology and Infectious Diseases Department, Hospital Universitario A Coruña, Spain. 2Clover Bioanalytical Software, Spain.
Evaluation of a novel method for detection of carbapenem hydrolysis with an automated software (Clover BioSoft) by MALDI-TOF MS | 30th European Congress of Clinical Microbiology & Infectious Diseases (ECCMID 2020) | Eva Gato1, Gema Méndez2, Luis Mancera2, Germán Bou1, Marina Oviaño1 | 1Clinical Microbiology and Infectious Diseases Department, Hospital Universitario A Coruña, Spain. 2Clover Bioanalytical Software, Spain.
Detection of Vancomycin-Resistant Enterococcus faecium Using MALDI-TOF Mass Spectrometry | Annual Congress in Clinical Mass Spectrometry (MSACL 2019) | Manuel Arroyo1, Adrián Ruiz2, Luis Mancera1, Lidia Quiroga2, Emilia Cercenado2, Belén Rodríguez Sánchez2 | 1Clover Bioanalytical Software, Spain. 2Hospital General Universitario Gregorio Marañón, Spain.
Use of MALDI-TOF Mass Spectrometry and Machine Learning to Detect the Adulteration of Extra Virgin Olive oils | American Society for Mass Spectrometry (ASMS 2018) | Simona Salivo1, Tom K. Abban1, Ismael Duque2, Luis Mancera2, Matthew E. Openshaw1 | 1Shimadzu, Manchester, UK. 2Clover Bioanalytical Software, Granada, Spain.
A Novel Software for the Characterization of Microorganisms Using Lipid Phenotyping and Statistical Analysis of MALDI-MS Data | American Society for Mass Spectrometry (ASMS 2018) | Gema Méndez-Cervantes1, Luis Mancera1, Gerald Stübiger2 | 1Clover Bioanalytical Software, Granada, Spain. 2Medical University of Vienna, Vienna, Austria.
Metrological characterization of the reproducibility of MALDI-TOF MS for bacterial strain typing | 28th European Congress of Clinical Microbiology & Infectious Diseases (ECCMID 2018) | Eloise Busby, Jim Hugget, Tim McHugh, Emmanuel Wey | University College London, UK.
Evaluation of critical parameters for the establishment of a clinical MALDI applications platform for liquid biopsy diagnostics | Accelerating Clinical Mass Spectrometry Europe (MSACL EU 2017) | Gerald Stubiger1, Emanuele Barborini1, Iris Prinz Werner1, Luis Mancera2, Michael Wuckzkowski1 | 1Medical University of Vienna, Austria. 2Clover Bioanalytical Software, Granada, Spain.
Blind Source Separation of MALDI Spectra from Noise by Sparse Representation in a Composite of Linear Bases | American Society for Mass Spectrometry (ASMS 2017) | Luis Mancera1, Luis González-Jaime1, Matthew Openshaw2 | 1Clover Bioanalytical Software, Granada, Spain. 2Shimadzu, Kratos Manchester, United Kingdom.
Dimensionality reduction of MALDI Imaging datasets using non-linear redundant wavelet transform-based representations | American Society for Mass Spectrometry (ASMS 2016) | Luis Mancera1, Lyna Sellami2, Jamie Cunliffe2, Luis González-Jaime1, Omar Belgacem2 | 1Clover Bioanalytical Software, Granada, Spain. 2Shimadzu, Kratos Manchester, United Kingdom.
MALDI mass spectrometry imaging in Alzheimer’s disease mouse model Het CRND8 (+/-) | American Society for Mass Spectrometry (ASMS 2016) | Lyna Sellami1, Marcia Roy2, Matthew E Openshaw11, Luis Mancera3, Omar Belgacem1 | 1Shimadzu, Kratos Manchester, United Kingdom. 2University of Edinburgh, Edinburgh, UK. 3Clover Bioanalytical Software, Granada, Spain.
Development of a novel MALDI-MS based liquid biopsy detection platform | CBMED Biomarker Research (CBMED 2016) |
Gerald Stubiger1, 2, Luis Mancera3, Omar Belgacem4 | 1Medical University of Vienna, Austria. 2CBmed GmbH – Center for Biomarker Research for Medicine, Graz, Austria. 3Clover Bioanalytical Software, Granada, Spain. 4Shimadzu, Manchester, United Kingdom.
Non-convex quasi-norm-based normalization of MALDI-MS Imaging Data | American Society for Mass Spectrometry (ASMS 2015) | Luis Mancera1, Lyna Sellami1, Philippa Hart1, Fiona Henderson2, Hervé Boutin2, Adam McMahon2, Omar Belgacem2 | 1Shimadzu, Manchester, UK. 2Wolfson Molecular Imaging Centre, University of Manchester, UK.